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red glifon 300 3000  (Addgene inc)


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    Addgene inc red glifon 300 3000
    Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red <t>Glifon</t> 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.
    Red Glifon 300 3000, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+glifon+300+3000/HL+2729+(%3DHL+716+%2B+rhyB+knockout_KanR)+(Bacterial+strain+%2330030)/pm40120584-267-16-24
    Average 93 stars, based on 4 article reviews
    red glifon 300 3000 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation."

    Article Title: Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation.

    Journal: Cell stem cell

    doi: 10.1016/j.stem.2025.02.017

    Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red Glifon 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.
    Figure Legend Snippet: Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red Glifon 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.

    Techniques Used: Staining, In Vitro, Quantitation Assay, Incubation, Expressing, Plasmid Preparation, Control, Transgenic Assay, Immunostaining, Marker, Two Tailed Test

    Related Articles

    Plasmid Preparation:

    Article Title: Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation.
    Article Snippet: Images were taken and processed using a Zeiss Axio Observer Z1 microscope with ApoTome.2 and Zeiss Axiovision software. .. The iGlucoSnFR sensor (a gift from J. Keller and L. Looger, Addgene plasmid # 164514) and Red Glifon 300/3000 (a gift from Takashi Tsuboi, Addgene plasmid # 163115; http://n2t.net/addgene:163115) glucose sensors were cloned into a lentiviral expression vector (pLEX) and transduced into human keratinocytes. ..

    Clone Assay:

    Article Title: Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation.
    Article Snippet: Images were taken and processed using a Zeiss Axio Observer Z1 microscope with ApoTome.2 and Zeiss Axiovision software. .. The iGlucoSnFR sensor (a gift from J. Keller and L. Looger, Addgene plasmid # 164514) and Red Glifon 300/3000 (a gift from Takashi Tsuboi, Addgene plasmid # 163115; http://n2t.net/addgene:163115) glucose sensors were cloned into a lentiviral expression vector (pLEX) and transduced into human keratinocytes. ..

    Expressing:

    Article Title: Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation.
    Article Snippet: Images were taken and processed using a Zeiss Axio Observer Z1 microscope with ApoTome.2 and Zeiss Axiovision software. .. The iGlucoSnFR sensor (a gift from J. Keller and L. Looger, Addgene plasmid # 164514) and Red Glifon 300/3000 (a gift from Takashi Tsuboi, Addgene plasmid # 163115; http://n2t.net/addgene:163115) glucose sensors were cloned into a lentiviral expression vector (pLEX) and transduced into human keratinocytes. ..



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    Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red <t>Glifon</t> 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.
    Red Glifon 300 3000, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+glifon+300+3000/HL+2729+(%3DHL+716+%2B+rhyB+knockout_KanR)+(Bacterial+strain+%2330030)/pm40120584-267-16-24
    Average 93 stars, based on 1 article reviews
    red glifon 300 3000 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    red glifon 300 163115 red glifon 3000 163116 red glifon nega 163117  (Addgene inc)
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    Addgene inc red glifon 300 163115 red glifon 3000 163116 red glifon nega 163117
    Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red <t>Glifon</t> 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.
    Red Glifon 300 163115 Red Glifon 3000 163116 Red Glifon Nega 163117, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+glifon+300+3000/xfz2%2FpCS2%2B+(Plasmid+%2316311)/pm34197723-207-10-9
    Average 93 stars, based on 1 article reviews
    red glifon 300 163115 red glifon 3000 163116 red glifon nega 163117 - by Bioz Stars, 2026-09
    93/100 stars
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    Image Search Results


    Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red Glifon 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.

    Journal: Cell stem cell

    Article Title: Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation.

    doi: 10.1016/j.stem.2025.02.017

    Figure Lengend Snippet: Figure 1. Intracellular glucose accumulates in keratinocyte differentiation (A) Heatmap representing average fold change of 614 metabolites identified in metabolomics, in early (day 3) and late (day 6) differentiation compared with the undifferentiated state (n = 5 biological replicates). (B) Accumulation of 2-NBDG (green), a fluorescent glucose analog, in differentiated keratinocytes with Hoechst nuclear stain (blue). Progenitor (PG) represents undifferentiated cells versus days 3 and 6 of calcium-induced differentiation in vitro (representative image of 3 independent samples, scale bar, 10 mm). (C and D) Quantitation of glucose-14C (C6) (C) or glucose-14C (C1) (D) accumulation during differentiation in culture, after 7 day incubation, normalized to mg of protein per sample (n = 3 biological replicates, 3 technical replicates per experiment). (E) Quantitation of glucose concentrations in regenerated epidermal organoid tissue, and whole epidermis undergoing stratification through day 6 analyzed (n = 3 biological replicates). (F) Epidermal organoids expressing vector control or the Red Glifon 3000 glucose sensor, and the dsRed signal (red) indicates detection of free glucose, with nuclear DAPI (blue) and phalloidin (green) stains (representative image of 3 biological replicates, scale bar, 50 mm). (G) Quantitation of dsRed intensity in the basal and spinous layers of the tissue normalized to phalloidin (n = 8 fields of view across 2 biological replicates). (H) Epidermis from transgenic mice expressing Red Glifon 3000; detection of free glucose (red), nuclear DAPI stain (blue), and immunostaining of differentiation marker keratin 10 (green); and gray bar indicates region of interest (ROI) used for quantitation in (I) (representative image shown, n = 3 biological replicates, scale bar, 50 mm). (I) Average fluorescence intensity with standard error (shaded region) in the ROI of Red Glifon, DAPI, and K10 across the epidermis from the basal layer up to but excluding the stratum corneum (n = 6). Data are represented as mean ± SEM, two-tailed unpaired Student’s t test was used for statistical analysis, *p < 0.05, ****p < 0.001. See also Figure S1 and Table S1.

    Article Snippet: The iGlucoSnFR sensor (a gift from J. Keller and L. Looger, Addgene plasmid # 164514) and Red Glifon 300/3000 (a gift from Takashi Tsuboi, Addgene plasmid # 163115; http://n2t.net/addgene:163115) glucose sensors were cloned into a lentiviral expression vector (pLEX) and transduced into human keratinocytes.

    Techniques: Staining, In Vitro, Quantitation Assay, Incubation, Expressing, Plasmid Preparation, Control, Transgenic Assay, Immunostaining, Marker, Two Tailed Test